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PROJECT
Presentation
The project
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The territory
Objectives
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METHODOLOGY
Studied habitats and taxons
Collection and selection of seeds
Identification and characterization
Processing of material
Dehydration
Conditioning and storage
Germination protocols
 
References

 
Treatment of plant material


Once in the bank, seeds are placed under quarantine to avoid contamination from insects, fungi or parasites. Seeds are then separated from the fruit, cleaned and submitted to some preliminary tests regarding seed quality. For those less known species a bibliography survey is required to collect those previously published data regarding seed anatomy and morphology, data that will be complemented with the direct study of our seeds under the stereomicroscope. In order to decide the treatment and storage conditions to be applied to the seeds, the first test to be carried out is moisture content. This test will enable us to include the seed in one of the main storage categories: orthodox, intermediate or recalcitrant, and therefore to anticipate their behaviour with respect to desiccation and temperature tolerance.

   
Post-maturation:Accessions are placed in storage rooms to allow a mild dehydration and a physiological maturation to take place (© 2005 Pablo Ferrer - CIEF; L. Podda - CCB).


All the above-mentioned procedures require the utilization of a high number of seeds. In those cases where the seeds available are scarce, a previous nursery work is required. In this case seeds are germinated in the laboratory and transferred to a nursery where plantlets are grown under controlled conditions until they are able to flower and produce new seed that will then be used in the germination assay.


   
The laboratory of the seedbank (to the left) is the ideal place for the preparation of germination tests, analysis of the viability of the lots, and for all the other seed handling operations (© 2005 Pietro Pavone e Giovanni Sanfilippo - DBUC ).


All activities carried out on seed lots are documented into specific work files, both on paper and in electronic format. These files become the historic memory of each accession, covering data from harvest to regeneration in the nursery, the protocols compiled, the quantitative and qualitative data, and the results obtained. These files should allow the updating of the bank database and encourage information and protocol exchange among the Institutions involved.

     
Photo (from left to right): seed germination in the laboratory; growth chamber and nursery
(© 2005 Pietro Pavone - DBUC; Institut des Régions Arides).

Sources:
Royal Botanic Gardens Kew - A Field Manual for Seed Collectors. Seed Collecting for the Millenium Seed Bank Project. Royal Botanic Garden Kew. Wakehurst Place - link
APAT - Manuale per la raccolta, studio, conservazione e gestione del germoplasma (versione 8.0) (IT)

 
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