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| Treatment of plant
material |
Once in the bank, seeds are placed under quarantine to
avoid contamination from insects, fungi or parasites.
Seeds are then separated from the fruit, cleaned and submitted
to some preliminary tests regarding seed quality. For
those less known species a bibliography survey is required
to collect those previously published data regarding seed
anatomy and morphology, data that will be complemented
with the direct study of our seeds under the stereomicroscope.
In order to decide the treatment and storage conditions
to be applied to the seeds, the first test to be carried
out is moisture content. This test will enable us to include
the seed in one of the main storage categories: orthodox,
intermediate or recalcitrant, and therefore to anticipate
their behaviour with respect to desiccation and temperature
tolerance.
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| Post-maturation:Accessions
are placed in storage rooms to allow a mild dehydration
and a physiological maturation to take place (©
2005 Pablo Ferrer - CIEF; L. Podda - CCB). |
All the above-mentioned procedures require the utilization
of a high number of seeds. In those cases where the seeds
available are scarce, a previous nursery work is required.
In this case seeds are germinated in the laboratory and
transferred to a nursery where plantlets are grown under
controlled conditions until they are able to flower and
produce new seed that will then be used in the germination
assay.
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laboratory of the seedbank (to the left) is the ideal
place for the preparation of germination tests, analysis
of the viability of the lots, and for all the other
seed handling operations (© 2005 Pietro Pavone
e Giovanni Sanfilippo - DBUC ). |
All activities carried out on seed lots
are documented into specific work files, both on paper and
in electronic format. These files become the historic memory
of each accession, covering data from harvest to regeneration
in the nursery, the protocols compiled, the quantitative
and qualitative data, and the results obtained. These files
should allow the updating of the bank database and encourage
information and protocol exchange among the Institutions
involved.
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Photo
(from left to right): seed germination in the laboratory;
growth chamber and nursery
(© 2005 Pietro Pavone - DBUC; Institut des Régions
Arides).
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| Sources:
Royal Botanic Gardens Kew - A Field Manual for Seed Collectors.
Seed Collecting for the Millenium Seed Bank Project. Royal
Botanic Garden Kew. Wakehurst Place - link
APAT - Manuale per la raccolta, studio, conservazione e gestione
del germoplasma (versione 8.0) (IT) |
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