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PROJECT
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METHODOLOGY
Studied habitats and taxons
Collection and selection of seeds
Identification and characterization
Processing of material
Dehydration
Conditioning and storage
Germination protocols
 
References

 
Seed collection


Seed collection is a key task requiring careful planning.
A correct planning should arise from the previously gathered information regarding the species and its distribution. Collection areas are thus selected in such a way that the highest genetic variability is ensured.
Collectors should verify in the field the identity of the taxa and the estimated collection date prior to scheduling the collecting.


Field studies (to the left) and plant material collections constitute essential procedures that have to be accomplished by specialized people (© 2005 L. Podda - CCB; Núria Membrives - IJBB; IRA)


If plants are to be used in reforestation or reintroduced in their original habitat it is essential for seed sampling to faithfully represent the genetic variability originally present in the habitat. Seed collectors should observe that sampling must be carried out covering more than 50% of individuals present but never taking more than 20% of available seeds. In the case of small populations, and mainly with rare or endangered species, collectors must avoid sampling a high number of individuals. In this case it is desirable to collect a smaller number of seeds and repeat the collecting.


     
Hypericum kelleri : plant, fruits and seeds. Threatened Cretian endenmic, whose germoplasma is conserved in the Seed Bank of the Mediterranean Agronomic Institute of Chania (Crete), partner in the GENMEDOC project . (© 2005 C.Fournaraki - MAICh).
 
 

In order to determine the proper time of harvest it is possible to use the “cut test”, which involves the opening of the seed along its main axes to observe the inside. The presence of turgid tissues with the coloration characteristic of the species indicates the optimal stage of maturity of the seed and the appropriateness of collection. In general seeds for collection must be non-damaged, viable and having reached maturity, just when they are ready for natural dispersion.
The collected genetic material, and the corresponding in-situ filled out collecting record, are taken to the bank where seeds are cleaned and treated before being included in the collection.
All these operations need much attention and care taken to maintain a high level of quality and viability in the seed bank.


     
The operations for the evaluation of material that enters the bank require patience and precision; seedlots with dead or infected seeds have to be avoided in order to obtain a satisfactory level of quality and viability in the collection (© 2005 Pietro Pavone - DBUC ).

Sources:
Royal Botanic Gardens Kew - A Field Manual for Seed Collectors. Seed Collecting for the Millenium Seed Bank Project. Royal Botanic Garden Kew. Wakehurst Place - link
APAT - Manuale per la raccolta, studio, conservazione e gestione del germoplasma (versione 8.0) (IT)

 
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