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PROJECT
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METHODOLOGY
Studied habitats and taxons
Collection and selection of seeds
Identification and characterization
Processing of material
Dehydration
Conditioning and storage
Germination protocols
 
References

 
Dehydration


Dehydration of seeds is a key feature in the management of accessions in the bank. Dehydration may last several months and require considerable knowledge of the seed morphology and anatomy and also of those physiological parameters related to seed development and maturity.
Seed dehydration is normally carried out in rooms under controlled temperature (10-20ºC) and humidity (10-15%).


 

Photo (left): Juniperus oxycedrus L. subsp. macrocarpa – fruits and seeds (orthodox) (© 2005 Josep Bonet - JBS); Castanea sativa - fruits and seeds (recalcitrant) (© 2006 David Fenwick).

Dehydration is the most delicate procedure of all those normally conducted in a seed bank, and also one of the most difficult problems to solve regarding ex situ conservation. Moisture loss should be carried out steadily because the different stages of seed development are affected by mechanical, chemical, morphological and physiological factors that may well enhance germination but may induce dormancy as well. Each type of seed tolerates a different dehydration process that should be properly conducted, otherwise seeds may lose their viability.

 

Photo: (left) drying room with silica gel, a chemical product that absorbs and reduces the moisture content of the seeds to values that ensure its conservation in mid- and long-term (Probert, 2003); (right) seed weighing to monitor the dehydration procedure (© 2005 MCarmen Martinell and Mònica Casanovas - IJBB)

Sources:
APAT - Manuale per la raccolta, studio, conservazione e gestione del germoplasma (versione 8.0)

 
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