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PROJECT
Presentation
The project
Partners
The territory
Objectives
Social fall out
Conclusions
 
METHODOLOGY
Studied habitats and taxons
Collection and selection of seeds
Identification and characterization
Processing of material
Dehydration
Conditioning and storage
Germination protocols
 
References

 
Packaging and storage


After the dehydration procedure, seeds are packaged in hermetically closed containers (aluminium envelopes, glass jars, flame-sealed glass tubes, vials of pyrex glass) to ensure long-term conservation. A small amount of silica gel is also placed within the container: the change of its colour in the presence of humidity allows periodical controls of the dehydration degree of the seeds.


Photo:(left) hermetical and transparent vials which permit the control of seed conservation conditions by avoiding unnecessary handling; (right) alternative solution to silica gel: it can be replaced by a paper label, bands of which are imbibed in a silica gel solution whose colour changes according to moisture percentage (© 2005 Pietro Pavone - DBUC; E. Mattana - BG-SAR, CCB).


The accessions that are packaged in this way, are directed to the:

- active collection – conservation at temperatures between 0 and 10oC; collection constituted by seedlots that will be used within a short or medium time span for in situ conservation activities (reinforcement of natural populations or re-introduction), direct sowing in the field, activities for ex situ conservation (propagation, exchanges with Index Seminum, etc.) or for the various tests and analyses in the laboratory

- base collection – conservation in cold rooms at a temperature of -18°C or inferior (IPBGR, 1985); dedicated to long-term conservation of seeds; these seedlots will only be used for periodical controls, every 5-10 years.




The base collection is conserved in cold rooms where seeds remain viable for several years. Photo: (left) the Banc de Llavors Forestals Generalitat Valenciana (Spain) and the Seed Bank of Catania (Italy). (© 2005 Pietro Pavone).


As in all the other procedures, seed storage necessitates careful management and tidy handling to avoid loss of material, time and information. Some fundamental rules have to be followed:

- careful planning for a better use of the available space;

- use of numbered labels and particular codes for recognition;

- constant control of the equipment in order to act quickly in case of breakdown.

Sources:
APAT - Manuale per la raccolta, studio, conservazione e gestione del germoplasma (versione 8.0) (IT)

 
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